Device

Part:BBa_K1627005:Design

Designed by: Alejandro Gutierrez   Group: iGEM15_Austin_UTexas   (2015-09-10)


Device to demethylate theobromine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 644
    Illegal NheI site found at 839
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1595
    Illegal BglII site found at 2091
    Illegal BglII site found at 2562
    Illegal XhoI site found at 3703
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4401
    Illegal AgeI site found at 2230
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

These genes were amplified from the pDCAF3 plasmid (BBa_K734000). An EcoRI restriction site was removed from the ndmB gene during the cloning process. The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly.


Source

The ndmB, ndmC, and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.

References